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Leukoagglutinin Test (Leukocyte Antibody)

Leukoagglutinin Test, are antibodies that agglutinate white blood cells in the blood. The leukoagglution is also called Leukocyte antibody. This reaction is thought to be due to the patient forming antibodies against the white blood cells of the donor blood of donner plasma containing Leukoagglutinin antibodies it will react with the recipient’s white blood cells and produce a febrile nonhemolytic reaction. Antibodies to Leukocyte or white blood cells may also develop in multiply transfused patients and multiparous women. Transfusion reactions may occur even though compatible blood is transfused. They are often accompanied by fever and in severe cases multi-organ system failure.

Other names Of This Test:

Leukoagglutinin Blood Level Test.

Symptoms due to Leukoagglutinin in Recipient:

The clinical indications of performing the Leukoagglutinin Blood Test include investigation. possible transfusion a reaction that may have the following signs or symptoms.

  • Chills
  • Fever
  • Skin flushing
  • Discolored Urine
  • Hypertension
  • Dyspnea
  • Cyanosis
  • Hypotension Mild WBCs decrease
  • Agglutination of Neutrophils seen

How To perform This Test:

Blood Sample Taken.


Following the specimen collection process for Leukoagglutinin Blood Test:

A band wrapped around the arm, a Superficial vein that lies within the claypit. The required amount of blood sample is collected by pulling the plunger of the syringe out slowly. Blood is immediately transferred into the blood container, which has the appropriate preservative Collect at least 3 to 5ml of blood in a Red Top Tube. Centrifuge the blood sample and collect the serum. The patient’s serum is incubated with leukocyte suspensions from four donors. Agglutination of any of the Leukocyte suspensions by the patient’s serum indicates the presence of granulocyte antibodies.

Result Interpretation:

Microscopic detection of white Blood cells that have taken up the dye is interpreted as positive value for the Leukoagglutinin blood test. This may point to a diagnoses of a transfusion reaction.

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