Dry Heat and Moist Heat Sterilization
Dry Heat and Moist Heat Sterilization, For ‘moist’ heat, temperatures of approximately 121–129 °C with pressure are used, whereas ‘dry’ heat requires temperatures from 176 to 232 °C for a longer duration.
| Maist Heat Sterilization | Dry Heat Sterilization | Maist heat Sterilization |
| Definition | A physical method of Sterilization that uses high temperature under dry conditions | A physical form of sterilization that uses a high temperature, Pressure generated by vaporized water e. Steam |
| Type of heat used | Day heat | Mast heat |
| Penetration Power | Less Penetration Power | More Penetration Power |
| Equipment used | Hot air ove | Autoclave |
| Sterilizing agent | Dry heat or Hot air | Saturated Steam under Pressure |
| Temperature and time | (160-180° C / 2-3h) (150-190°C / 0.5-3h) | 121°C/151bs,fsi 15-20 min |
| Mode of Action | Oxidative damage of biomolecules | (110-135°C / 15-45min) Denaturation and coagulation of Proteins |
| Biological waste decontamination | No | Yes |
| Indicator organism | Bacillus atrophaeus formerly Bacillus subtilis | Geobacillus Stearothermophilus |
| Examples | Deep frying Grilling Boiling Baking | Steaming Stewing Poaching Most effective |
| Type of materials | Glassware: Petri plates Pipettes, coverslips test tubes, slides, Surgical/metal instruments: Forceps Scissors Needles Ails, Powders. waxes etc: Fabrics, rubber or Plastic ware. (not used) | Heat Stable Liquids: (microbiological media, chemical solutions water) Glassware: Petri plates Pipettes Test tubes Plastic ware Surgical Instruments (Textile material Rubber etc). Not used |
| Equipment cost | Less Expensive | More Expensive |
| Types | Two types: 1. Static- Air Type. 2. forced-Air Type. | 1. Autoclaving. 2. Pasteurization. 3. Tyndallization. |
MCQ’s:
(a moist heat sterilization
(b) Hot Sterilization
(c) None
(d) Air Sterilization
(a) Rubber
(b) Moist heat sterilization
(c) Dry heat sterilization
(d) Oven sterilization
Day heat sterilization has -effective?
(a) Less effective
(b) more effective
(c) both
(d) none
Use of Physical method with high temperature>
(a) Maoist heat sterilization
(b) Dry heat sterilization
(c) both or a, b
(d) None of above
Moist heat sterilization equipment cost?
(a) Most expensive
(b) Less expensive
(c) Beth
(d) None of above
FAQ?
FAQ? is a document (as on a website) that provides answers to a list of typical questions that users might ask regarding a particular subject.
Question No 1:
What is day heat sterilization?
Answer:
Dry heat sterilization is the physical method of sterilization that uses at high temperatures under day conditions. It is an object that is one of the earliest forms of sterilization Practiced.
Question No 2:
What is the sterilization of moist heat?
Answer:
Sterilization by moist heat is also known as steam sterilization. It destroys micro-organisms in a Product with steam under pressure. Moist heat sterilization is a Physical method that uses high-temperature Pressure generated by temperature.
Question No 3:
Give Examples of Dry and Moist Heat Sterilization.?
Answer:
Examples of Dry heat sterilization
Boiling
Deep Frying
Grilling
Baking
Examples of moist heat Sterilization
Steaming
Stewing
Poaching
Pan frying
Question No 4:
What are the two main types of Dry heat sterilization?
Answer:
There are two main types of Dry-heat Sterilization.
Static-Air:
The static an type is referred to as the oven type sterilizer as heating Coils in the bottom of the Unit cause the hot air to rise the chamber Via gravity convection.
Forced-Air:
A forced-air central heating system is one which uses air as its heat transfer medium.
Question No 5:
Mention the indicate that used in Day and Moist heat sterilization?
Answer:
1- Dry heat sterilization:
Bacillus atrophies formerly Bacillus Subtilis.
2- Moist heat sterilization:
Geobacillus Stearothermophilus
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