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Dry Heat and Moist Heat Sterilization

Dry Heat and Moist Heat Sterilization, For ‘moist’ heat, temperatures of approximately 121–129 °C with pressure are used, whereas ‘dry’ heat requires temperatures from 176 to 232 °C for a longer duration.

Maist Heat SterilizationDry Heat SterilizationMaist heat Sterilization
DefinitionA physical method of Sterilization that uses high temperature under dry conditionsA physical form of sterilization that uses a high temperature, Pressure generated by vaporized water e. Steam
Type of heat usedDay heatMast heat
Penetration PowerLess Penetration PowerMore Penetration Power
Equipment usedHot air oveAutoclave
Sterilizing agentDry heat or Hot airSaturated Steam under Pressure
Temperature and time(160-180° C / 2-3h)
(150-190°C / 0.5-3h)
15-20 min
Mode of ActionOxidative damage of biomolecules(110-135°C / 15-45min) Denaturation and coagulation of Proteins
Biological waste decontaminationNoYes
Indicator organismBacillus atrophaeus formerly Bacillus subtilisGeobacillus Stearothermophilus
ExamplesDeep frying
Most effective
Type of materialsGlassware:
Petri plates Pipettes, coverslips test tubes, slides,
Surgical/metal instruments:
Ails, Powders. waxes etc:
Fabrics, rubber or Plastic ware. (not used)
Heat Stable Liquids:
(microbiological media, chemical solutions water)
Petri plates
Test tubes
Plastic ware Surgical Instruments
(Textile material Rubber etc).
Not used
Equipment costLess ExpensiveMore Expensive
TypesTwo types:
1. Static- Air Type.
2. forced-Air Type.
1. Autoclaving.
2. Pasteurization.
3. Tyndallization.


(a moist heat sterilization

(b) Hot Sterilization

(c) None

(d) Air Sterilization

(a) Rubber

(b) Moist heat sterilization

(c) Dry heat sterilization

(d) Oven sterilization

Day heat sterilization has -effective?

(a) Less effective

(b) more effective

(c) both

(d) none

Use of Physical method with high temperature>

(a) Maoist heat sterilization

(b) Dry heat sterilization

(c) both or a, b

(d) None of above

Moist heat sterilization equipment cost?

(a) Most expensive

(b) Less expensive

(c) Beth

(d) None of above


FAQ? is a document (as on a website) that provides answers to a list of typical questions that users might ask regarding a particular subject.

Question No 1:

What is day heat sterilization?


Dry heat sterilization is the physical method of sterilization that uses at high temperatures under day conditions. It is an object that is one of the earliest forms of sterilization Practiced.

Question No 2:

What is the sterilization of moist heat?


Sterilization by moist heat is also known as steam sterilization. It destroys micro-organisms in a Product with steam under pressure. Moist heat sterilization is a Physical method that uses high-temperature Pressure generated by temperature.

Question No 3:

Give Examples of Dry and Moist Heat Sterilization.?


Examples of Dry heat sterilization 

Deep Frying

Examples of moist heat Sterilization 

Pan frying

Question No 4:

What are the two main types of Dry heat sterilization?


There are two main types of Dry-heat Sterilization.


The static an type is referred to as the oven type sterilizer as heating Coils in the bottom of the Unit cause the hot air to rise the chamber Via gravity convection.


A forced-air central heating system is one which uses air as its heat transfer medium.

Question No 5:

Mention the indicate that used in Day and Moist heat sterilization?


1- Dry heat sterilization: 

Bacillus atrophies formerly Bacillus Subtilis. 

2- Moist heat sterilization: 

Geobacillus Stearothermophilus

By Mehfooz Ali

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6 thoughts on “Dry Heat and Moist Heat Sterilization”
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