Creatinine Test in Blood

Creatinine Test in Blood

Creatinine Test in Blood, The serum creatinine test measures the amount of creatinine in the blood. Serum Creatinin is a waste product, Produced in the muscles, after creatinin breakdown. Creatinine is organic and helps to supply energy to muscle cells. It is synthesized mainly in the liver from arginine, glycine, and methionine. Once formed creatinine is not reused in the body’s metabolism and thus functions solely as a waste Product. The more muscle a person has more creatinine they Produce. A creatinine test measures how well your kidneys on Performing their job of filtering waste from your body’s blood.

Principle of Creatinin Test:

The main Principle of the creatinine test is it can be determined in serum or urine.

Jaffe’s Reaction:

Jaff’s reaction where the creatinine Produces an orange color with Picric acid in an alkaline medium.

Other Names of Creatinin Test in Blood:

Also, known Also called Serum Creatinine, Plasma Canalinin, and Blood Creatinine.

Reference Range of Creatinin in Blood:

Between Infants female & malesValue / RangeUnit
aLess than 2 years0.1 – 0.4mg/dL
b2 years to < 6 years0.2 – 0.5mg/dL
c6 years to 10 years0.3 – 0.6mg/dL
d10 years to < 18 years0.4 – 1.0mg/dL
eFemales 18 years or older0.5 – 1.0mg/dL
fMales 18 years or older0.6 – 1.2mg/dL

Causes of High Creatinine, Seen in Blood:

Low level of Creatinin, Causes in Blood:

Diagnostic Importance:

→ To screen a suspected disorder involving muscle
→ To diagnose kidney disease
→ To evaluate kidney function
→ Screen Glomerular filtration
→ Creatinine is a more reliable indicator of renal function

How to Perform the Test?

  • By using a Blood sample (In a vial)
  • By using a urine sample (In Urine Container)
  • Chemical Tests Through Reagents or Buffers
  • Microscopic Examination (Through microscope)
  • Manual Performance

Sample Precautions:

Serum/Plasma: There is no need for any Preparation.

Manual Procedure:

R-1500 μL500 μL
R-2500 μL500 μL
Mix and incubate for 5 minutes:
Standard100 μL
Serum100 μL

First reading after 30 seconds and reading after to second out 520nm.

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5 thoughts on “Creatinine Test in Blood”
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