Creatinine Phosphokinase
Creatinine Phosphokinase is a test that measure the creatinine kinase CK in your blood. This test called Creatinine Kinase or creatinine phosphokinase. Creatinine kinase is a type of protein. It is found in the following parts of the body.
- Heart
- Muscles
- Liver
- Testiest
A very small amount of this enzyme is found in the brain and then in the blood. Its level in the blood is controlled by the liver. Creatine is present in the blood. It is actually derived from the dephosphorylating of creatine phosphate. Creatine is the end product of protein metabolism and is excreted in urine and the appropriate pH for this reaction should be 6.8 – 9.0. Phosphate is formed only when the amount of energy ATP is very high. For example, contraction begins within a resting muscle. And when there is a shortage of ATP, the stored creatine phosphate is quickly broken down and ATP is formed. Creatinine Phosphokinase is found in large amounts in skeletal muscle, in the brain, in the thyroid, and in the lungs, where this enzyme has significant function.
Is This Have other Name:
Creatinine Kinase, Creatinine Phosphokinase, CK.
How The Test Performed:
A Blood Sample is needed.
Why do I need a CK test?
You may need a CK test if you have symptoms of a muscular disorder. These include:
- Muscle pain and/or cramps
- Muscle weakness
- Balance problems
- Numbness or tingling
- You may also need this test if you had a muscle injury or stroke. CK levels may not peak until up to two days after certain injuries, so you may need to be tested a few times.
Clinical interpretation:
The normal range of CPK in serum is -24. The amount of CPK in serum is about 2/3 in women compared to men. It is released into the muscle and it should be noted that the total serum CPK level is elevated even after mild trauma. For example, by walking fast, injecting drugs.
CPK is increased in the following diseases:
- Sugar
- In trauma within the muscles
- Exercise a lot
- By intramuscular injection
CPK levels may also increase in brain tissue. In addition, there are other clinical conditions in which CPK levels are elevated,
- Meningitis
- Encephalitis
- In the hepatic region
Principle:
Nowadays, there are many automations which make it very easy to perform tests. But I give you the manual method.
| Control | Sample | |
| Enzymes/ Co-Enzyme Substrate | 1.00 ml | 1.00 ml |
| Sample | ——– | 200 ul |
| Control | 200 ul | ——– |
Take two cuvettes and mark them as test and control. Then mix them and incubate for one minute at 37°C and take first, second and third readings.
What do the results mean?
If your results show you have a higher than normal level of CK, it may mean you have an injury or disease of the muscles, heart, or brain.
[…] cells. It is synthesized mainly in the liver from arginine, glycine, and methionine. Once formed creatinine is not reused in the body’s metabolism and thus functions solely as a waste Product. The more muscle a person has more creatinine they […]
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[…] alkaline picrate calorimetric method is used. In this method, chemical components other than creatinine are not affected and it is considered to be a more effective method. The total test time with this method is 15 minutes while the total […]