Heller's Test

Heller’s Test

Heller’s Test is a biochemical test performed to detect proteins in a sample by the denaturation of these proteins by the addition of strong acids. It is a qualitative test for the detection of protein in the urine.

Principle of Heller’s Test:

The test is based on the principle of precipitation of proteins, Concentrated Nitric Acid Denature protein, and forming a white precipitate when urine comes in contact with the Nitric Acid.

Symptoms Of Heller’s Test:

Affected children show clinically significant the following terms:

  • Child Hood disintegrative disorder (CDP).
  • Social function.
  • Development delays in language.

Requirements for Heller’s Test:

  • Concentrated Nitric Acid,
  • Dropper, Test Tubes,
  • Test tube stand,
  • Urine (5ml),
  • Pipettes,


Take 2ml of concentrated nitric and in a test tube Add urine drop by drop by the side of the test tube. The appearance of white ring at the junction indicates the presence of protein Because nitric acid is a denatured protein and a white ring is formed at the junction. So this mean Test is positive for protein in the urine.


Positive Result:

The positive test is represented by the formation of a white ring at the junction of the two distinct layers, It indicates the presence of proteins in the given sample.

Negative Result:

A negative result is represented by the absence of a white ring. This indicates the absence of protein in the sample.

Heller's Test


The concentrated and used in the test is Corrosive and thus, should be handled carefully The test is qualitative and doesn’t give the concentration of protein present in the sample.

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