Bile Pigments
Bile Pigments, The bile pigments are formed by the decomposition of the porphyrin ring and contain a chain of four pyrrole rings. Bilirubin, for example, the brownish-yellow pigment that gives feces its characteristic color, is the end product of the breakdown of heme from destroyed red blood cells.
- Bilirubin: Unconjugated:
- Indicator: water insoluble
- Bilirubin: Conjugated
- Direct: Water-soluble
Fouchet’s Test:
Principle:
Bile pigments absorbed on ppt of Basoy. Ferric chloride in Fouchet’s reagent oxidizes bilirubin into green biliverdin.
Interference:
Procedure:
Take 5ml of urine + 1ml MgSoy +2ml 10%.
Backe Filter and the precipitate few drops of Fouchet’s reagents.
Observation:
Color changes from yellow to Pista green.
Interpretation: Hay’s Sulphur test/Fouchel’s Test:
| Type of Jaundice | Hemolytic Jaundice Pre hapatic | Hepatocellular Jaundice (Hepatic) | obstructive Jaundice Poste (Hepatic) |
| Bile salts | Absent | + | ++ |
| Bile Pigments | Absent | ++ | +++ |