Microtomes, To view human tissue in a microtome, it is necessary to take enough sections of the tissue through which light can pass easily. The thickness of the tissue section should be uniform. The instruments used for this purpose are called microtomes.
Different types of microtomes are available in the market but all types must have the following features.
- Support should be present to firmly hold the tissue block.
- The tissue block or blade should be moved forward after each cut.
- A tissue block or one of the splints should be fixed, while the other object moves.
Types of Different Microtomes Used in Pathology Lab:
- Base Sledge Microtome
- Cambridge Rocking Microtome
A microtome is a tool used in the laboratory to cut thin slices of samples/sections of different objects so that these slices of different samples can be examined through a microscope can be done.
Types of Microtomes:
In this microtome, the sample is placed in a fixed holder (Fixed Holder) which moves forward and backward across the chamber and divides the sample into slices.
It is a simple basic microtome that works by rotary action and produces slices from the sample by rotary action.
This microtome is used to cut frozen samples in a liquid nitrogen chamber.
It works by rotating blades to extract slices from the sample with less pressure than normal blades.
This hard material (Hard Material) for example, Bones & Teeth, is used to make slices.
This microtome is used for Contact Press Slicing. It can be used without sample embedding, freezing, freezing, and chemical fixing.
Their types depend on the profile of the blade. For example:
- Planner Concave shaped
- Wedge Shaped
- Chisel Shaped
- Biconcave Knife
- Tool Edge Profile Knife
- Disposable Knife
- Disposable Blades